Abstract

Abstract. Electrolyzed oxidizing (EO) water, produced by the electrolysis of separating a weak sodium chloride solution into alkaline and acidic components, has the potential to be used as a cleaning and sanitizing agent for Clean-In-Place (CIP) cleaning of on-farm milking systems. To demonstrate this, a pilot-scale pipeline milking system was constructed and soiled using raw milk inoculated with four common microorganisms found in milk. The milking system was then washed with alkaline EO water followed by acidic EO water according to the experimental design by using surface response methodology. After cleaning, the effectiveness of the EO water treatment was evaluated by ATP bioluminescence and microbiological analysis through enrichment culture. The effect of different temperatures of the alkaline and acidic EO water were investigated and optimized. By determining the logarithmic mean temperature for the treatment for both the alkaline and acidic EO water and by comparing values obtained from conventional heat transfer calculation versus the actual experimental data, a generalized mathematical model was built as a set of algebraic equations in order to determine the effective treatment temperatures for different configurations of the milking equipment based on the maximum reduction in ATP bioluminescence values. The model thus obtained was validated for the pilot scale milking system through validation and comparison studies

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