Maternal exposure to Bisphenol‐A Induces Epigenetic Changes in metabolic syndrome markers of Offspring

Abstract

Environmental factors exert significant effects of on disease progression later in the life, when exposed in‐utero. Bisphenol A (BPA) is a monomer of plastic products widely used in everyday life. BPA shows hormone mimicking properties, and its adverse effects on endocrine glands and hormones may affect developmental embryology of offspring. Bisphenol A may possibly have an epigenetic role in onset and progression of several adult diseases related to metabolic system. To determine the epigenetic effects of in‐utero Bisphenol A on markers related to metabolic syndrome in mice offspring. This study was conducted at department of Anatomy Liaquat University of Medical & Health Sciences Jamshoro and Animal house of Sindh Agriculture University Tando Jam Pakistan after Ethical approval. Adult female BALB/c strains mice were divided in three groups each comprised of 10 female mice– G1 (controls), G2 (ethanol 0.10 ml i.p) and G3 (BPA 10 mg i.p). Animals were housed individually in polypropylene cages, in standard lighting, controlled temperature and humidity conditions. Control group was fed ad libitum. After mating, the positive vaginal plug was checked to confirm pregnancy. Offspring body weight, behavior, movement, blood glucose; serum insulin, cholesterol and HDLc were measured at 5 months age and at 15 months age before sacrifice of animals. Pancreas, kidney, adipose tissue and uterine tissue were stained with H & E staining and Immunohistochemistry and examined under microscopy. Data was analyzed on SPSS 22.0 (at 95% confidence interval). Offspring of group G3 reveals abnormal changes in behavior and movement. Body weight, blood glucose, serum insulin, cholesterol and HDLc were high compared to controls (P=0.0001). Islets of Lanherghan's, Kidney and Uterine tissue reveals abnormal anatomical changes with anti‐ Islet‐1 Ig, Ksp cadherin, and anti‐ MLH antibody respectively. In utero exposure of endocrine disruptor Bisphenol A exerts Diabetogenic and Atherogenic effects with abnormal immunohistochemistry changes in endocrine pancreas, kidney and uterus.Support or Funding InformationThis study was funded by Liaquat University of Medical & Health Sciences, Jamshoro, PakistanThis abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.