Maternal exposure of triclosan cause intrauterine development restriction, delay in puberty installation and deregulation of testicular function in rat offspring

Camila Stacheski Machado,João Paulo De Arruda Amorim,Elaine Manoela Porto Amorim,Michael Aparecido Machado,Romário Willian Welter

doi:10.4025/actascihealthsci.v41i1.37929

Camila Stacheski Machado, João Paulo De Arruda Amorim + Show 3 more

Open Access

https://doi.org/10.4025/actascihealthsci.v41i1.37929

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Abstract

This study aimed to evaluate the effects of maternal exposure to triclosan (TCS), during gestation and lactation on physical development, puberty installation and testicular function of male offspring in puberty and sexual maturity. Sixteen pregnant Wistar rats were used and divided into four experimental groups: GI- they received corn oil daily by gavage; GII- they received 75 mg kg -1 day -1 of TCS; GIII- they received 150 mg kg -1 day -1 of TCS and GIV- they received 300 mg kg -1 day -1 of TCS during pregnancy and lactation. The mean weight of the treated rat’s offspring was lower in comparison to GI group. In the exposed offspring. A delay in puberty installation was observed following the exposure to triclosan. Regarding to the stages of the seminiferous epithelium in puberty, it was noticed an increase of the I-VI stages in GIV group, compared with to GI. In sexual maturity, it was observed an increase of VII-VIII, IX-XIII in contrast to a reduction of XIV stage in treated animals, comparing to GI. There was no difference in the number of Sertoli cells. We conclude that the maternal exposure to TCS during gestation and lactation causes restriction of the intrauterine development, delay in the puberty installation and deregulation in the seminiferous epithelium cycle.

Highlights

The experimental procedures are in accordance with the Ethical Principles in Animal Research adopted by the Brazilian College of Animal Experimentation (BCAE) and approved by the Unioestes Ethics Committee for Animal Experimentation and Practical Classes
The body weight gain in the end of the lactation period was lower in the rats of the Group IV (GIV) group, when compared to Group I (GI) group
The endocrine system performs a crucial role in the homeostasis maintenance of the organism and it is frequently affected by exogenous stimulus

Summary

Introduction

The triclosan (2,4,4'-trichloro-2'-hydroxydiphenyl ether) (TCS) is a bactericide agent of a wide spectrum. It is often used in pharmaceutical industry of personal hygiene products such as antiseptic, toothpaste, soaps, cosmetics, cleaning products, besides toys and textile products (Eui-Man, Beum-Soo, Kyung-Chul, & Eui-Bae, 2012; Honkisz, Zieba-Przybylska, & Wojtowicz, 2012; Axelstad, Boberg, Vinggaard, Christiansen, & Hass, 2013). The TCS quantification on plasma, breast milk and urine in human beings (Zorrilla et al, 2009; Paul, Hedge, DeVito, & Crofton, 2010) confirms its use in large scale and the human exposure to this substance. The biological half-life on plasma and urine is of 21 and 11 hours, respectively, this composed, fat-soluble, suffers bioaccumulation in the adipose tissue (Honkisz et al, 2012)

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