Abstract
Introduction: Preeclampsia has been one of the leading causes of maternal death in Indonesia. It is postulated that its relationship with oxidative stress may be the underlying pathology of the disease. Nutrients and amino acids have been suggested as a scavenger for oxygen-free radicals. No previous study regarding the amino acid status in preeclampsia has been conducted in women in Indonesia. Methods: This was a cross-sectional study of a total of 64 pregnant women, 30 with normal pregnancy and 34 with severe preeclampsia. Data were obtained in Cipto Mangunkusumo National Referral Hospital in Jakarta from July to December 2020. Maternal blood samples were taken during or soon after delivery. Amino acid levels were analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Bivariate analysis was then performed. Results: We identified 19 different levels of amino acids in this study. Four amino acids that were elevated in the preeclampsia group were phenylalanine, serine, glycine, and glutamate. Serine (331.55 vs. 287.43; p = 0.03), glycine (183.3 vs. 234.35, p = 0.03), and glutamate levels (102.23 vs. 160.70, p = 0.000) were higher in preeclamptic patients. While in the essential amino acids group, phenylalanine levels (71.5 vs. 85.5, p = 0.023) were higher, and methionine levels (16.3 vs. 12.9, p = 0.022) were lower in preeclamptic patients. Conclusions: These findings suggest that severe preeclampsia had differences in concentration of some amino acids compared to normal pregnancy. Glutamate and methionine were associated with preeclampsia. Furthermore, a more detailed study regarding amino acids in the pathomechanism of preeclampsia is suggested.
Highlights
Preeclampsia has been one of the leading causes of maternal death in Indonesia
We found that most subjects in both normal and preeclampsia groups had intakes that were below the Estimated Average Requirement and recommended dietary allowances (RDA) recommendations of 1.1 g/kg body weight per day [16]
Our findings suggest that severe preeclampsia had significant differences in the concentrations of some amino acids compared to normal pregnancy
Summary
Preeclampsia has been one of the leading causes of maternal death in Indonesia. It is postulated that its relationship with oxidative stress may be the underlying pathology of the disease. No previous study regarding the amino acid status in preeclampsia has been conducted in women in Indonesia. Results: We identified 19 different levels of amino acids in this study. Four amino acids that were elevated in the preeclampsia group were phenylalanine, serine, glycine, and glutamate. While in the essential amino acids group, phenylalanine levels (71.5 vs 85.5, p = 0.023) were higher, and methionine levels (16.3 vs 12.9, p = 0.022) were lower in preeclamptic patients. Conclusions: These findings suggest that severe preeclampsia had differences in concentration of some amino acids compared to normal pregnancy. A more detailed study regarding amino acids in the pathomechanism of preeclampsia is suggested. Pregnancy is complicated by oxidative stress from maternal decidua and placental villus. Insufficient decidualization may lead to placental hypoxia and, endothelial and vascular dysfunction manifested as preeclampsia [4]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
