Mate tea-mediated reduction in catecholamine synthesis improves cutaneous wound healing of chronically stressed mice

Abstract

Chronic stress stimulates oxidant production and oxidative damage which compromise cutaneous wound healing. Mate tea rich is in antioxidant compounds and may be a good alternative for treatment of oxidative diseases. Therefore, the aim of this study was to investigate the effects of supplementation with mate tea on cutaneous healing in stressed mice. Mice were submitted to rotational stress (ST) and treated with mate tea (MT) daily until euthanasia. An excisional lesion was created on each mouse and 4 or 10days later, the lesions were analyzed. In addition, murine skin fibroblasts were exposed to elevated epinephrine (E) levels plus mate tea, and fibroblast activity was evaluated. In in vivo assays, the supplementation with mate tea inhibited the stress-induced increase in: catecholamine synthesis (ST: 23.3±2.5; ST+MT: 18.0±0.9ng/μl, p<0.05), carbonyl protein content (ST: 962.0±35.6; ST+MT: 35.7±8.9nmol/μg protein, p<0.05), lipid peroxide levels (ST: 18.5±2.3; ST+MT: 11.8±1.2nmol/mg protein, p<0.05), wound contraction (ST: 44±4; ST+MT: 17±2%, p<0.05), re-epithelialization (ST: 908±35; ST+MT: 2081±138μm, p<0.05), transforming growth factor-β (ST: 5.0±0.02; ST+MT: 1.3±0.06 u.a., p<0.05), and myofibroblast density (ST: 19±2; ST+MT: 9±1%, p<0.05). Stress-induced reduction in the amount of macrophages (ST: 133±19; ST+MT: 392±33 cells, p<0.05) and neutrophils (ST: 1161±62; ST+MT: 1313±103 cells, p<0.05) and hydroxyproline levels (ST: 1.3±0.2; ST+MT: 4.6±0.9ng/mg tissue, p<0.05), and this was reversed by mate tea. In in vitro assays, the treatment with mate tea reversed the reduction in collagen deposition (E: 1.7±0.3; E+MT: 3.7±0.01pixels, p<0.05) and the increase in cell proliferation (E: 331±2; E+MT: 203±3% of control, p<0.05), accumulation of lipid peroxides (E: 0.66±0.009; E+MT: 0.55±0.01nmol/mg protein, p<0.05), and increase of tenascin-C protein expression (E: 3.8±0.004; E+MT: 3.4±0.004a.u., p<0.05) induced by epinephrine. In conclusion, the supplementation with mate tea improves the cutaneous wound healing of chronically stressed mice.