Abstract
Background. Angiogenesis is a complex process involved in both growth and progression of several human and animal tumours. Tryptase is a serin protease stored in mast cells granules, which plays a role in tumour angiogenesis. Mast cells (MCs) can release tryptase following c-Kit receptor (c-KitR) activation. Method. In a series of 25 gastric cancer patients with stage T3N2-3M0 (by AJCC for Gastric Cancer 7th Edition), immunohistochemistry and image analysis methods were employed to evaluate in the tumour tissue the correlation between the number of mast cells positive to tryptase (MCPT), c-KitR expressing cells (c-KitR-EC), and microvascular density (MVD). Results. Data demonstrated a positive correlation between MCPT, c-KitR-EC, and MVD to each other. In tumour tissue the mean number of MCPT was 15, the mean number of c-KitR-EC was 20, and the mean number of MVD was 20. The Pearson test correlating MCPT and MVD, c-KitR-EC and MVD was significantly (r = 0.64, P = 0.001; r = 0.66, P = 0.041, resp.). Conclusion. In this pilot study, we suggest that MCPT and c-KitR-EC play a role in gastric cancer angiogenesis, so we think that several c-KitR or tryptase inhibitors such as gabexate mesilate and nafamostat mesilate might be evaluated in clinical trials as a new antiangiogenetic approach.
Highlights
Angiogenesis is a complex process involved in growth, invasion, and metastasis of several animal and human tumours [1,2,3]
Immunohistochemical staining by antibodies anti-tryptase, anti-c-Kit receptor (c-KitR) and anti-CD31 allows the demonstration that mast cells positive to tryptase (MCPT) and c-KitR-EC are well recognizable in highly vascularized gastric carcinoma tissue (Figure 1)
Due to the possible interobserver variability at light microscopy in the evaluation of MCPT, c-KitR-EC, and microvascular density (MVD), the counts were performed by mean the above image analysis system
Summary
Angiogenesis is a complex process involved in growth, invasion, and metastasis of several animal and human tumours [1,2,3]. No data have been published regarding the correlation between MCs positive to tryptase (MCPT), c-Kit receptor expressing cells (c-KitR-EC), and microvascular density (MVD) in gastric carcinoma tissue. We have evaluated correlations between the number of MCPT, c-KitR-EC, and MVD in a series of 25 gastric carcinomas with stage T3N2-3M0 (by AJCC for Gastric Cancer 7th Edition), by means of immunohistochemistry and image analysis methods. In a series of 25 gastric cancer patients with stage T3N2-3M0 (by AJCC for Gastric Cancer 7th Edition), immunohistochemistry and image analysis methods were employed to evaluate in the tumour tissue the correlation between the number of mast cells positive to tryptase (MCPT), c-KitR expressing cells (c-KitR-EC), and microvascular density (MVD). We suggest that MCPT and c-KitR-EC play a role in gastric cancer angiogenesis, so we think that several c-KitR or tryptase inhibitors such as gabexate mesilate and nafamostat mesilate might be evaluated in clinical trials as a new antiangiogenetic approach
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