Mast Cells and Collagen Fibrillogenesis in Zero Gravity Conditions

V V Shishkina,D A Atiakshin

doi:10.18499/2225-7357-2019-8-3-79-88

Abstract

The aim of research was to study participation of skin mast cells of C57BL/6J mice in the mechanisms of fibrillogenesis under zero gravity conditions.Material and methods. A histochemical study of the skin from the lateral thigh of the C57BL/6J mice exposed aboard the International Space Station for 21–24 days, as well as animals of the control groups – vivarium, baseline and ground, – was performed. Mast cells were detected with an assessment of the secretome participation in collagen fibrillogenesis after protocols of separate and combined histochemical staining with Giemsa’s solution and silver impregnation. Microsections were analyzed using a hardware-software complex based on a ZEISS Axio Imager. A2 research microscope (Carl Zeiss, Germany).Results. Zero-gravity conditions led to a change in the activity of mast cells degranulation and histotopographic localization, a decrease in the content of large granules, a decrease in the cooperation with fibroblast/ fibrocyte and the intensity of fibrillogenesis, a decrease in co-localization with the reticular fibers of the extracellular matrix of the skin tissue, as well as a modification of the intrapopulation interaction. The article discusses possible molecular-cellular causes of changes in the activity of fibrillogenesis and polymerization of tropocollagen molecules into supramolecular fibrous structures in the skin connective tissue aboard the International Space Station.Conclusion. Zero gravity conditions caused an increase in the secretory activity of mast cells in the skin, simulated the processes of intercellular signaling with other representatives of the specific tissue microenvironment, and resulted in the weakening of collagen fibrillogenesis.

Highlights

A histochemical study of the skin from the lateral thigh of the C57BL/6J mice exposed aboard the International Space Station for 21–24 days, as well as animals of the control groups – vivarium, baseline and ground, – was performed
Mast cells were detected with an assessment of the secretome participation in collagen fibrillogenesis after protocols of separate and combined histochemical staining with Giemsa’s solution and silver impregnation
Zero-gravity conditions led to a change in the activity of mast cells degranulation and histotopographic localization, a decrease in the content of large granules, a decrease in the cooperation with fibroblast/fibrocyte and the intensity of fibrillogenesis, a decrease in co-localization with the reticular fibers of the extracellular matrix of the skin tissue, as well as a modification of the intrapopulation interaction

Summary

Introduction

Проводили гистохимическое исследование кожи латеральной поверхности бедра мышей линии C57BL/6J, экспонированных в течение 21–24 сут на борту Международной космической станции, а также животных контрольных групп – виварийного, базального и наземного. Условия невесомости приводили к активизации либерализации компонентов секретома ТК во внеклеточный матрикс (количество дегранулированных форм достоверно возрастало до 61.5±3.3% по сравнению с показателями контрольных групп), изменению гистотопографической локализации, уменьшению интрацитоплазматического содержания крупных гранул, снижению кооперации с клетками фибробластического дифферона и интенсивности фибриллогенеза, урежению солокализации с ретикулярными волокнами дермы кожи, а также к модификации внутрипопуляционного взаимодействия. Обсуждаются возможные молекулярно-клеточные причины изменения активности фибриллогенеза и полимеризации молекул тропоколлагена в надмолекулярные волокнистые структуры в соединительной ткани кожи на борту Международной космической станции. Пребывание в невесомости вызывало возрастание секреторной активности тучных клеток кожи, моделировало процессы межклеточного сигналлинга с другими представителями специфического тканевого микроокружения и приводило к ослаблению фибриллогенеза коллагена. The aim of research was to study participation of skin mast cells of C57BL/6J mice in the mechanisms of fibrillogenesis under zero gravity conditions

Methods

Results

Conclusion