Mass transfer of nanobubble aeration and its effect on biofilm growth: Microbial activity and structural properties

Abstract

It is necessary to improve the performance and reduce the aeration cost is of wastewater treatment by aerobic biofilm systems. Nanobubble aeration is supposed to be a promising method to achieve these goals. Compared with coarse bubbles, dissolved oxygen profiling showed that the nanobubbles provided more oxygen to biofilms, offering superior oxygen supply capacity and 1.5 times higher oxygen transfer efficiency. Nanobubble aeration accelerated the growth of the biofilm and achieved better removal efficiencies of chemical oxygen demand and ammonia, with as maximum as six times higher dehydrogenase activity, and more extracellular polymeric substance content than when using the traditional aeration mode. This is attributed to the enhancement of metabolism and the proliferation of microorganisms. Confocal laser-scanning microscopy imaging confirmed that nanobubble aeration affected the components of biofilm by shifting the microbial community and changing its metabolic pathways of biofilms, such as carbohydrate synthesis. Nanobubble aeration resulted in an energy saving of approximately 80%. The assessment of nanobubble aerated biofilm growth suggests that this technique can offer a rapid-initiation, high efficiency, and low-cost strategy for aerobic biofilm systems in wastewater treatment.