Abstract
Inositol pyrophosphates are cellular signals that are created by the actions of inositol kinases and are degraded by highly active inositol phosphatases. The potent actions of these phosphatases suggest these signals must be created near their sites of action. To identify sites where the inositol kinase, PPIP5K1 acts, we performed affinity purification of PPIP5K1 from HEK293 cells and analyzed these samples using mass spectrometry to identify the proteins pesent (10.1016/j.cellsig.2016.02.002) [1]. We further decreased PPIP5K1 levels in HeLa cells and treated these with PPIP5K1 siRNA. We then monitored the motility of these cells in Scratch assays.
Highlights
HeLa cells were treated with PPIP5K1 siRNA PPIP5K1 and co-purified proteins were analyzed and identified using mass spectrometry
Reports cellular interactions with PPIP5K1 The data contributes to the interaction network of the cell and cell signaling The data may further stimulate investigations of inositol signaling in cell motility
Data The data in this article is the analysis of proteomic data of the interactions between PPIP5K1 and other cellular proteins [1]
Summary
Movie files Mass spectroscopy, Microscope (Operetta High Content microscope (Perkin Elmer, Woodbridge, ON, Canada)) Filtered Affinity-tagged PPIP5K1 was purified from cultured HEK293 cells. HeLa cells were treated with PPIP5K1 siRNA PPIP5K1 and co-purified proteins were analyzed and identified using mass spectrometry. HeLa cells treated with PPIP5K siRNA were subjected to a Scratch test and their motility was monitored by video Montreal, Canada. Reports cellular interactions with PPIP5K1 The data contributes to the interaction network of the cell and cell signaling The data may further stimulate investigations of inositol signaling in cell motility. The data in this article is the analysis of proteomic data of the interactions between PPIP5K1 and other cellular proteins [1]. Included here are videos of the motility of HeLa cells with normal and decreased levels of PPIP5K1
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
