Abstract
AbstractAn unambiguous differentiation between leucine and isoleucine residues in peptides is possible with unimolecular metastable ion (MI) and collisional activation (CA) spectra. The immonium ion \documentclass{article}\pagestyle{empty}\begin{document}$ {\rm [Y}\!\mathop {\rm N}\limits^{\rm + } {\rm H = CHC}_{\rm 4} {\rm H}_{\rm 9} {\rm ]} $\end{document}, which is present in the normal mass spectra of all leucine or isoleucine containing peptides, yields MI spectra with which this differentiation can be made with high sensitivity. The MI and CA spectra of other peptide fragments are also applicable in particular cases. MI and CA spectra can also be used to obtain information on the amino acide sequences of individual ions. In general more extensive sequece information is available from CA than from MI spectra. This information appears to be particularly promising for the sequencing of oligopeptide mixtures or of ions produced by field or chemical ionization.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.