Abstract
Shoot tips and axillary buds of cultivar ‘Rosamini’ were cultured on MS (Murashige and Skoog, 1962) medium plus 30 g l −1 sucrose, 8 g l −1 agar, supplemented with 1 mg l −1 benzylaminopurine and 10 −4 mg l −1 indol butyric acid (IBA). Multiplication rates of six- to seven-fold were reached when shoots were subcultured every 4 weeks. Multiplication rates decreased with prolonged maintenance of shoots without subculture. Root induction was achieved on half strength MS plus 20 g l −1 sucrose, 6 g l −1 agar, supplemented with 1 mg l −1 IBA. Root elongation was performed on the same medium without IBA. The effect of indolacetic and naphthaleneacetic acids, different agar concentrations, and of several MS salt dilutions on rooting and the influence of the type of culture vessel on multiplication and rooting steps were studied. Flower bud break occurred in early stage after transfer of rooted plants to pots, and occasionally occurred in culture vessels.
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