Abstract
The results of the recent years researches support the need for personalized therapeutic of cancer by completing the clinical, imagistic and histopathological diagnosis with molecular studies to identify new useful biomarkers for diagnosis, prognosis and tumor progression. Maspin is a non-inhibitory serine protease having a proapoptotic activity, suppressor of tumor invasion, metastasis and angiogenesis. Ezrin is a member of Ezrin/Radixin/Moesin (ERM) family, involved in cellular adhesion mechanisms, motility and invasiveness of tumor cells. In colorectal tumors, there is a heterogeneity of research results regarding the clinical significance of the maspin due to a possible partnership with other molecules with which it interacts through the same signaling pathways. Our study investigated the two molecule�s immunoreactivity (IR) in 92 colorectal tumors highlighting an inverse correlation between ezrin�s and maspin�s expression, suggesting the fact that ezrin�s overexpression could influence maspin�s tumoral suppressor role. Furthermore there was observed a difference of the molecules IR within the same tumoral stage, suggesting their utility regarding the treatment protocol of these tumors.
Highlights
The results of the recent years researches support the need for personalized therapeutic of cancer by completing the clinical, imagistic and histopathological diagnosis with molecular studies to identify new useful biomarkers for diagnosis, prognosis and tumor progression
At molecular level, has some specific features that support its inclusion in the non-inhibitory serine protease category: the presence of a hydrophobic reactive site loop (RSL) shorter than that of the rest of the serpins, which is thought to decrease the stability of the molecule and justify the inability to make the transition from stressed to relaxed state
A series of studies associate its overexpression with the metastatic process and the poor prognostic for digestive system tumors, HNSCC, osteosarcomas, gynecological tumors [12] while studies of Andersson et al, Palou et al, Moilanen et al, Palmerini et al have opposite results [13-16]. In view of these aspects the present study evaluated ezrin immunohistochemical expression correlated with maspin‘s expression in colorectal carcinomas in order to highlight possible interrelationships between these two molecules and to indentify a possible molecular pattern specific to colorectal cancer
Summary
PAVEL ONOFREI1*, ELENA CARMEN COTRUTZ1*, ANA EMANUELA BOTEZ1, VASILE BOGDAN GRECU1, CARMEN SOLCAN2, ANCA ILEANA SIN3, DANIELA CRISTINA DIMITRIU4, PAUL DAN SIRBU5, VASILE LUCIAN BOICULESE6, VLAD PORUMB7, LAURA STOICA1*, DOINITA TEMELIE OLINICI1 1Grigore T. Maspin‘s crystal structure analysis has identified a new domain specific to it - the G á helix area or the P1 position of the RSL that is capable of presenting a relaxed/stressed configuration by redistributing the charged residues This conformational modification of the molecule‘s region is specific to maspin, and research indicates that this domain is important for binding molecules from the extracellular matrix (ex: á1 and â1 integrin), being essential for maspin‘s involvement in cell migration [5,6]. A series of studies associate its overexpression with the metastatic process and the poor prognostic for digestive system tumors, HNSCC, osteosarcomas, gynecological tumors [12] while studies of Andersson et al, Palou et al, Moilanen et al, Palmerini et al have opposite results [13-16] In view of these aspects the present study evaluated ezrin immunohistochemical expression correlated with maspin‘s expression in colorectal carcinomas in order to highlight possible interrelationships between these two molecules and to indentify a possible molecular pattern specific to colorectal cancer. Statistical analysis Associations between clinical-pathological variables (tissue type, tumor stage, dissemination, and presence of metastases) were analyzed using Chi-square tests and the occurrence frequency of events was compared using IBM SPSS Version 18.0 for Windows (SPSS Inc, Chicago, IL)
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