MARKERS OFENDOGENOUSINTOXICATION AND OXIDATIVESTRESSIN PATIENTS WITH OPIOID DEPENDENCES

Abstract

Aim of study is to evaluate changes in fluorescence indicators that reflect the endotoxicosis and oxidative stress status in patients with opioid dependence syndrome through treatment. Materials and methods. The study involved 28 patients with opioid dependence syndrome and 20 healthy persons. Fluorescence parameters reflecting the accumulation of proteins oxidative modification products were determined in the blood plasma. Results and discussion. At the stage of admitting the patients to the hospital, the blood plasma was found to contain increased levels of FLOPs and bityrosine residues by 18–30% against 12–20% reduction of the intensity of intrinsic and probe fluorescence of proteins. At the same time, upon the completion of the therapy course (15–17 days) all the studied parameters returned to the values those in the control group, except for the level of bityrosine residues, which remained 20% above that in the control. This may be accounted for by the process of updating plasma proteins and the greater stability demonstrated by bityrosine molecules. Conclusion. FLOPs and tyrosine residue identification can be used for laboratory monitoring of early response to the therapy as well as for a long-term efficiency follow-up in patients with opioid dependence syndrome.