Abstract
Screening for the source of virus resistance in horticultural plants or specific characterization as hybridization, through symptoms, requires time and depends on the weather and knowledge of plant characteristics. So, it is important to develop specific gene markers to allow rapid diagnosis by PCR. Markers were developed based on sequences homology comparison of susceptible and resistant plants provided by HORTEC SEEDS in tomato for Tomato yellow leaf curl virus (TYLCV) by the resistance gene Ty-1, in zucchini for Zucchini yellow mosaic virus (ZYMV) and Papaya ringspot virus estirpe watermelon (PRSV-W), and in lettuce for Lettuce mosaic virus (LMV). Fragments of 249 bp were amplified only by resistant plants to TYLCV as the hybrids 2648 and Aguamiel, and not for varieties as Santa Cruz or Carina. It were observed for ZYMV the amplification of 791 bp by the resistant hybrid Px7051 and not for the susceptible cultivar La Belle; for PRSV-W using the same zucchini plants the amplification of 650 bp for susceptible and 750 bp for resistant; for LMV the 421 bp amplification only for the resistant cultivar Brasil 303 and not for susceptible Baba de Verao. Finally, it was observed that primers PK47F/R were able to check the Cabotia seed hybrids of pumpkin Jabras.
Highlights
Since the major diseases of horticultural plants as tomato (Solanum lycopersicum L.), zucchini (Cucurbita pepo L.) and lettuce (Lactuca sativa L.) are caused by virus, whose high biological diversity demands resistant material as the best way of disease controlling, the development of specific primers for identification of resistance genes will allow the resistance source screening by a common PCR, in order to facilitate the incorporation of desirable genes.The begomovirus Tomato yellow leaf curl disease (TYLCD) is one of the main limiting factors for tomato production [1], which belongs to the monopartite Tomato yellow leaf curl virus (TYLCV)
Markers were developed based on sequences homology comparison of susceptible and resistant plants provided by HORTEC SEEDS in tomato for Tomato yellow leaf curl vírus (TYLCV) by the resistance gene Ty-1, in zucchini for Zucchini yellow mosaic virus (ZYMV) and Papaya ringspot viruses tirpe watermelon (PRSV-W), and in lettuce for Lettuce mosaic virus (LMV)
It were observed for ZYMV the amplification of 791 bp by the resistant hybrid Px7051 and not for the susceptible cultivar La Belle; for PRSV-W using the same zucchini plants the amplification of 650 bp for susceptible and 750 bp for resistant; for LMV the 421 bp amplification only for the resistant cultivar Brasil 303 and not for susceptible Babá de Verão
Summary
Since the major diseases of horticultural plants as tomato (Solanum lycopersicum L.), zucchini (Cucurbita pepo L.) and lettuce (Lactuca sativa L.) are caused by virus, whose high biological diversity demands resistant material as the best way of disease controlling, the development of specific primers for identification of resistance genes will allow the resistance source screening by a common PCR, in order to facilitate the incorporation of desirable genes. The accessions and inbred lines that carry the Ty-1 gene present high levels of resistance for monopartite (TYLCV) or bipartite types such as Tomato severe rugose virus (ToSRV); the SCAR (Sequence Characterized Amplified Region) marker that does not need restriction by enzyme, called C-19, was developed by the ToSRV evaluation in tomato [3]. By the central region sequencing of eIF4E cDNAs from tolerant, susceptible and resistant lettuce genotypes to LMV (GenBank AF530162), three types of alleles, Ls-eIF4E1, Ls-eIF4E2 and Ls-eIF4Ewere found and using RT-PCR was reported the eIF4E-PagI CAPS marker (generated with PagI restriction of Ls-eIF4E cDNA) [14]. Specific marker that allows the knowledge of the desirable genotype with a simple PCR reaction is important to aid the improvement work of horticultural plants such as tomato, zucchini, pumpkin and lettuce
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