Abstract
Two genes, CsLRR-RPK2 (CsGy5G015660) and CsaMLO8 (Csa5G623470), have been considered as powdery mildew (PM) resistance genes in cucumbers. In this study, we evaluated the involvement of the alleles of these two genes in PM resistance in 100 commercial Korean cucumber inbred lines. To achieve this, we developed cleaved amplified polymorphic sequences (CAPS) and InDel markers from CsLRR-RPK2 and CsaMLO8. Genotyping analysis indicated that the CsLRR-RPK2-CAPS marker showed a stronger correlation with the PM-resistant phenotype, with an 84% consistency compared to the CsaMLO8-InDel marker. The use of the CsaMLO8-InDel marker showed a 70% consistency between phenotype and genotype results. It was proposed that the CsLRR-RPK2-CAPS marker successfully eliminated PM-susceptible inbred lines, since both genotype and phenotype results were 100% identical. Furthermore, the present study revealed that the introduction of one of these alleles is probably enough to confer PM resistance in cucumbers. However, seven PM-resistant inbred lines harbored either CsaMLO8 or CsLRR-RPK2 alleles, indicating that there is another PM-resistant resource(s) besides CsaMLO8- and CsLRR-RPK2–originated resistance in the commercial Korean inbred lines. Our results provide reliable evidence confirming two PM-resistant candidate genes for the detection of PM resistance resources in cucumber inbred lines.
Highlights
The cucumber [Cucumis sativus L. (2n = 2x = 14)] is a member of the Cucurbitaceae family, which is the same botanical family as melons and squashes [1]
We applied Powdery mildew (PM)-resistant molecular markers derived from two PM-resistant candidate genes (CsaMLO8 and CsLRR-RPK2) to 100 Korean inbred lines to evaluate the accuracy of marker-assisted selection
The evaluation of PM resistance and genotyping analysis using the markers for CsaMLO8 and CsLRR-RPK2 alleles in 100 Korean cucumber inbred lines is shown in Table 2 and Figure 3
Summary
(2n = 2x = 14)] is a member of the Cucurbitaceae family, which is the same botanical family as melons and squashes [1]. It is an economically important vegetable cultivated worldwide [2]. Powdery mildew (PM) is one of the most serious productivity and quality-loss-inducing diseases in cucumber-growing fields and greenhouses worldwide. The application of protective fungicides has been considered a vital way to control P. xanthii. The most efficient and environmentally friendly way to control the spread of P. xanthii is the breeding of PM-resistant cultivars [7]
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