Marker-assisted Backcrossing for Identification of Salt Tolerant Rice Lines

Ja Rashid,M Moniruzzaman,Mm Islam,Ms Islam,Sn Begum

doi:10.3329/ijarit.v2i2.14008

Abstract

SSR or microsatellite markers are proved to be ideal for making genetic maps, assisting selection and studying genetic diversity in germplasm. SSR markers are playing important role to identify gene for salt tolerance that can be helpful for plant breeders to develop new cultivars. The experiment was conducted during the period from July 2009 to November 2010 in the experimental field and Biotechnology Laboratory of Plant Breeding Division, Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh to identify salt tolerant rice line of BC1F1 progenies of Binadhan-5 x FL-478 using SSR markers. Salt tolerant genotype, FL-478 was crossed with high yielding variety, Binadhan-5. Randomly selected 40 BC1F1 progenies along with their two parents (Binadhan-5, FL-478 and F1) were genotyped with microsatellite or SSR markers for identification of salt tolerant rice lines. Parental polymorphism survey was assayed by 10 SSR markers and three polymorphic SSR markers viz., RM 336, RM 510, and RM 585 were selected to evaluate BC1F1 rice lines for salt tolerance. In respect of Primer RM 336, 11 lines were found as salt tolerant and 25 lines were heterozygous and 3 lines were susceptible. Primer RM 510 identified two tolerant, 14 heterozygous and 22 susceptible lines. And primer RM 585 identified 4 lines as tolerant and 35 lines as susceptible. Thus, these markers could be efficiently used in tagging salt tolerant genes, in marker-assisted selection and quantitative trait loci (QTL) mapping. The selected BC1F1 could be used for developing BC2F1 and BC2F2 and mapping genes for salinity tolerance. DOI: http://dx.doi.org/10.3329/ijarit.v2i2.14008 Int. J. Agril. Res. Innov. & Tech. 2 (2): 1-8, December, 2012

Highlights

Rice (Oryza sativa L.) is the most important food crop of the world
For the marker assisted backcrossing (MAB) the experiment was conducted during the period from July 2009 to November 2010 in the experimental field and Biotechnology Laboratory of Plant Breeding Division, Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh
Forty BC1F1 progenies were selected for this study from the backcross made between rice of F1 (Binadhan-5 x FL-478) and Binadhan-5

Summary

Introduction

Rice (Oryza sativa L.) is the most important food crop of the world. Eighty five per cent of it is devoted for human consumption (IRRI, 1997). Salt stress is a major problem in coastal areas because of seawater intrusion during high tide and the rising shallow saline groundwater, during the dry season. More than 54 million ha of rice land in Asia are affected by salinity. Salinity is one of the major obstacles in increasing production in rice growing areas worldwide, which is an ever-present threat to crop yield. Accumulation of salt in the soil has deleterious effects and leads to a reduction in crop production, including rice. Use of salttolerant variety considered the most economical and most effective way of increasing crop production on saline soils. Development of salt tolerant varieties considered as one of the strategies to increase rice production in saline prone coastal areas

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