Abstract
We introduce a method for marker-free cell discrimination based on optical tweezers. Cancerous, non-cancerous, and drug-treated cells could be distinguished by measuring the trapping forces using holographic optical tweezers. We present trapping force measurements on different cell lines: pre-B lymphocyte cells (BaF3; normal cells), their Bcr-Abl transformed counterparts (BaF3-p185; cancer cells) as a model for chronic myeloid leukaemia (CML) and Imatinib treated BaF3-p185 cells. The results are compared with reference measurements obtained by a commercial flow cytometry system.
Highlights
Discrimination of different cells and cell states is important for identification of diseases, treatment monitoring, and indication of drug response in clinical diagnosis and biomedical research
We have shown that holographic optical tweezers can be used to discriminate between normal, cancerous and drugtreated cancerous leucocytes without using additional markers
The optical force that a focused light beam exerts onto the cell seems to be more sensitive to changes of the cell than the standard FACS-based measurement with fluorescence-based markers
Summary
Discrimination of different cells and cell states is important for identification of diseases, treatment monitoring, and indication of drug response in clinical diagnosis and biomedical research. A lot of methods have been developed in the past for differentiating cells. Due to their high speed and the contactless operation, optical methods are especially useful for this purpose. Image-processing based techniques that classify different cells have already attained impressive results, but for many applications methods which purely rely on simple dyeing or phasecontrast techniques are not powerful enough. Discrimination based solely on images is not possible.
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