Marker controlled screening of resistant to red stele root rot (Rpf1 gene) strawberry selected forms

Alexander Lyzhin,Irina Luk’Yanchuk

doi:10.1051/bioconf/20213601006

Abstract

The results of the analysis of allelic polymorphism of the Rpf1 red stele root rot resistance gene in strawberry hybrid forms using diagnostic DNA markers were shown. The recessive homozygous genotype (rpf1rpf1) was identified in strawberry seedlings 932-29 (F. virginiana subsp. platypetala (Rydb.) Staudt × Feyerverk), 933-4 (F. virginiana subsp. platypetala (Rydb.) Staudt × Rubinovyy kulon), 62-7, 62-23 (Bylinnaya × Feyerverk), 65-2, 65-15 (Olimpiyskaya nadezhda × Bylinnaya) and 35-16 (922-67 × Maryshka). The heterozygous state of the Rpf1 gene was identified in strawberry forms 69-29 (Feyerverk × Bylinnaya), 62-41 (Bylinnaya × Feyerverk), 72-24, 72-71 (Privlekatelnaya × Bylinnaya), 65-17, 65-24 (Olimpiyskaya nadezhda × Bylinnaya), which allows them to be used in breeding for resistance to P. fragariae var. fragariae as valuable initial forms.

Highlights

Phytophthora fragariae var. fragariae Hickman is the causative agent of red stele root rot, one of the most dangerous strawberry fungal diseases [1, 2]
Resistance red stele root rot in strawberry varieties and forms is due to the presence of race-specific oligogens in the genome [6], of which the Rpf1, Rpf2, and Rpf3 genes make the greatest contribution to the formation of resistance [7]
The OPO-16C marker is identified in strawberry hybrid forms 932-29 (F. virginiana subsp. platypetala (Rydb.) Staudt × Feyerverk), 933-4 (F. virginiana subsp. platypetala (Rydb.) Staudt × Rubinovyy kulon), 62-7, 62-41 (Bylinnaya × Feyerverk), 72-24, 72-71 (Privlekatelnaya × Bylinnaya), 65-17, 65-24 (Olimpiyskaya nadezhda × Bylinnaya) and 69-29 (Feyerverk × Bylinnaya) (Figure 1., Table)

Summary

Introduction

Phytophthora fragariae var. fragariae Hickman is the causative agent of red stele root rot, one of the most dangerous strawberry fungal diseases [1, 2]. Fragariae Hickman is the causative agent of red stele root rot, one of the most dangerous strawberry fungal diseases [1, 2]. Resistance red stele root rot in strawberry varieties and forms is due to the presence of race-specific oligogens in the genome [6], of which the Rpf, Rpf, and Rpf genes make the greatest contribution to the formation of resistance [7]. Identification of genetic determinants of strawberry resistance to P. fragariae var. The purpose of this study was the analyze allelic polymorphism of the Rpf red stele root rot resistance gene in strawberry hybrid seedlings for identify and involve valuable forms in the breeding programs to create resistant to fungal pathogens strawberry genotypes

Objectives

Methods

Results