Marker-assisted selection for temporary maintainers of a recessive epistemic genic male sterility in Brassica napus L

Abstract

Four varieties of Brassica napus,Xiangyou 15,Huyou 15,You 88 and Huyou 17,were crossed with a temporary maintainer(TAM) named HYZ1-TAM respectively and produced four F2 populations.In these populations,molecular markers associated with Bnms3 and Bnrf were used to select new temporary maintainer plants which were identified by test-cross.Sixty-two plants with TAM marker-genotype were selected by marker-assisted selection(MAS) from three hundred and fifty-five plants of four F2 populations.Fifty-seven plants(with the accuracy of 91.94%) were TAM genotype proved by test-cross.Compared with traditional method,it is very efficient to select TAM by MAS.In the Bnms4 locus,all the four varieties have a same genotype which is ms4ms4.