Abstract
Two non-pigmented, motile, Gram-negative marine bacteria designated R9SW1T and A3d10T were isolated from sea water samples collected from Chazhma Bay, Gulf of Peter the Great, Sea of Japan, Pacific Ocean, Russia and St. Kilda Beach, Port Phillip Bay, the Tasman Sea, Pacific Ocean, respectively. Both organisms were found to grow between 4°C and 40°C, between pH 6 to 9, and are moderately halophilic, tolerating up to 20% (w/v) NaCl. Both strains were found to be able to degrade Tween 40 and 80, but only strain R9SW1T was found to be able to degrade starch. The major fatty acids were characteristic for the genus Marinobacter including C16:0, C16:1 ω7c, C18:1 ω9c and C18:1 ω7c. The G+C content of the DNA for strains R9SW1T and A3d10T were determined to be 57.1 mol% and 57.6 mol%, respectively. The two new strains share 97.6% of their 16S rRNA gene sequences, with 82.3% similarity in the average nucleotide identity (ANI), 19.8% similarity in the in silico genome-to-genome distance (GGD), 68.1% similarity in the average amino acid identity (AAI) of all conserved protein-coding genes, and 31 of the Karlin's genomic signature dissimilarity. A phylogenetic analysis showed that R9SW1T clusters with M. algicola DG893T sharing 99.40%, and A3d10T clusters with M. sediminum R65T sharing 99.53% of 16S rRNA gene sequence similarities. The results of the genomic and polyphasic taxonomic study, including genomic, genetic, phenotypic, chemotaxonomic and phylogenetic analyses based on the 16S rRNA, gyrB and rpoD gene sequence similarities, the analysis of the protein profiles generated using MALDI-TOF mass spectrometry, and DNA-DNA relatedness data, indicated that strains R9SW1T and A3d10T represent two novel species of the genus Marinobacter. The names Marinobacter salarius sp. nov., with the type strain R9SW1T ( = LMG 27497T = JCM 19399T = CIP 110588T = KMM 7502T) and Marinobacter similis sp. nov., with the type strain A3d10T ( = JCM 19398T = CIP 110589T = KMM 7501T), are proposed.
Highlights
IntroductionThe genus Marinobacter (family Alteromonadaceae, order Alteromonadales, class Gammaproteobacteria) was created by Gauthier et al for a hydrocarbon degrading bacterium
The genus Marinobacter was created by Gauthier et al for a hydrocarbon degrading bacterium
The objectives of this study were to classify two newly isolated marine bacteria; strain R9SW1T, which was derived from a water sample collected from Chazhma Bay (Gulf of Peter the Great, Sea of Japan, Pacific Ocean) during taxonomic studies of microbial communities developed in sea water contaminated by radionuclides [12]; and strain A3d10T, which was isolated from Port Philip Bay during the course of polymer biodegradation studies [13]
Summary
The genus Marinobacter (family Alteromonadaceae, order Alteromonadales, class Gammaproteobacteria) was created by Gauthier et al for a hydrocarbon degrading bacterium. At the time of writing, the genus comprises 33 validly described species, http://www.bacterio.net/marinobacter.html [1], which accommodates Gram-negative, chemoheterotrophic and halophilic, rodshaped bacteria [2,3]. Marinobacter are one of the dominant bacterial community groups constantly recovered from hydrocarbon polluted sites [8,9,10]. It was recently demonstrated that M. vinifirmus was able to effectively degrade toluene, benzene, ethylbenzene, and p-xylene [11]. The comparative taxonomic investigations of these bacteria, together with their close relatives, Species name
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