Mariniflexile ostreae sp. nov., a member of the family Flavobacteriaceae isolated from an oyster.

Abstract

A Gram-negative, aerobic, non-flagellated and rod-shaped bacterial strain able to move by gliding, designated TYO-10(T), was isolated from an oyster collected at Tongyoung on the South Sea, South Korea. Strain TYO-10(T) was found to grow optimally at 30 °C, at pH 7.0-8.0 and in the presence of 2.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain TYO-10(T) joined the cluster comprising the type strains of Mariniflexile species. Strain TYO-10(T) exhibited 16S rRNA gene sequence similarity values of 95.4-95.7 % to the type strains of Mariniflexile species and of less than 95.4 % to the type strains of other recognized species. Strain TYO-10(T) was found to contain MK-6 as the predominant menaquinone and iso-C15:0, iso-C15:1 G, anteiso-C15:0, iso-C17:0 3-OH and iso-C15:0 3-OH as the major fatty acids. The major polar lipids of strain TYO-10(T) were identified as phosphatidylethanolamine and an unidentified lipid, which is similar to those of the type strains of Mariniflexile species, but different from those of other phylogenetically related species. The DNA G+C content of strain TYO-10(T) was determined to be 35.9 mol%. The differential phenotypic properties, together with the phylogenetic distinctiveness, revealed that strain TYO-10(T) is separated from other Mariniflexile species. On the basis of the data presented, strain TYO-10(T) is considered to represent a novel species of the genus Mariniflexile, for which the name Mariniflexile ostreae sp. nov. is proposed. The type strain is TYO-10(T) (= KCTC 42113(T) = CECT 8622(T)).