Marine Exopolysaccharide Complexed With Scandium Aimed as Theranostic Agents.

Mattia Mazza,Pascal E Reiller,Cyrille Alliot,Sandrine Huclier-Markai,Sylvia Colliec-Jouault,Corinne Sinquin

doi:10.3390/molecules26041143

Abstract

(1) Background: Exopolysaccharide (EPS) derivatives, produced by Alteromonas infernus bacterium, showed anti-metastatic properties. They may represent a new class of ligands to be combined with theranostic radionuclides, such as 47Sc/44Sc. The goal of this work was to investigate the feasibility of such coupling. (2) Methods: EPSs, as well as heparin used as a drug reference, were characterized in terms of molar mass and dispersity using Asymmetrical Flow Field-Flow Fractionation coupled to Multi-Angle Light Scattering (AF4-MALS). The intrinsic viscosity of EPSs at different ionic strengths were measured in order to establish the conformation. To determine the stability constants of Sc with EPS and heparin, a Free-ion selective radiotracer extraction (FISRE) method has been used. (3) Results: AF4-MALS showed that radical depolymerization produces monodisperse EPSs, suitable for therapeutic use. EPS conformation exhibited a lower hydrodynamic volume for the highest ionic strengths. The resulting random-coiled conformation could affect the complexation with metal for high concentration. The LogK of Sc-EPS complexes have been determined and showing that they are comparable to the Sc-Hep. (4) Conclusions: EPSs are very promising to be coupled with the theranostic pair of scandium for Nuclear Medicine.

Highlights

The measured differential Refractive Index (dRI) values are plotted against the concentration trationand andfitted fittedtotoa alinear linearregression regression(Figure (Figure2), 2),the theslope slopeofofwhich whichcorresponds correspondstotothe the specificrefractive refractiveindex indexincrement incrementofofthe theanalyte analyteininthe thegiven givenexperimental experimentalconditions
The major interest in studying materials containing metal complexes of polymers stems from the fact that the rheological and other material properties of polypolymers stems from the fact that the rheological and other material properties of polymeric meric systems are strongly affected by complexation with metals [42]
A slightly sulfated named EPS-DR and a highly sulfated named -DRS, exhibiting heparin-like properties, have been characterized in terms of molar mass distribution and conformation. Their respective ∂n/∂c values were determined for being used with Asymetrical Flow Field-Flow Fractionation (AF4)-Multi Angle Light Scattering (MALS) for reaching their Mw values

Summary

Introduction

Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. Osteosarcoma is the most common primary malignant bone tumor in children and young adults. It is a very aggressive type of cancer with a low prognosis, but molecular mechanisms of metastases have been identified for designing new therapeutic strategies in the frame of personalized medicine. Glycoaminoglycans (GAGs) are a hallmark related to cancer growth and spread. GAGs could be employed in new drug delivery systems to deliver active compounds for therapeutic in oncology [1]

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