Abstract
Marine collagen peptides (MCPs) with the ability to promote cell proliferation and migration were obtained from the skin of Nibea japonica. The purpose of MCPs isolation was an attempt to convert the by-products of the marine product processing industry to high value-added items. MCPs were observed to contain many polypeptides with molecular weights ≤ 10 kDa and most amino acid residues were hydrophilic. MCPs (0.25–10 mg/mL) also exhibited 2, 2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl, superoxide anion, and 2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging activities. Furthermore, MCPs promoted the proliferation of NIH-3T3 cells. In vitro scratch assays indicated that MCPs significantly enhanced the scratch closure rate and promoted the migration of NIH-3T3 cells. To further determine the signaling mechanism of MCPs, western blotting was used to study the expression levels of nuclear factor kappa-B (NF-κB) p65, IκB kinase α (IKKα), and IκB kinase β (IKKβ) proteins of the NF-κB signaling pathway. Our results indicated protein levels of NF-κB p65, IKKα and IKKβ increased in MCPs-treated NIH-3T3 cells. In addition, MCPs increased the expression of epidermal growth factor (EGF), fibroblast growth factor (FGF), vascular endothelial growth factor (VEGF), and transforming growth factor (TGF-β) in NIH-3T3 cells. Therefore, MCPs, a by-product of N. japonica, exhibited potential wound healing abilities in vitro.
Highlights
Owing to the boom in marine product processing industry, there is a huge production of by-products that are either discarded or used as animal feed or fertilizer [1,2]
Components less than 1, 3, 5 and 10 kDa accounted for 55.25%, 79.29%, 85.71% and 90.31% of the spectrum respectively indicating these marine collagen peptides (MCPs) primarily contained a large number of low molecular weight polypeptides
MCPs prepared from the skin of N. japonica exhibited potential cell proliferation and migration activities
Summary
Owing to the boom in marine product processing industry, there is a huge production of by-products that are either discarded or used as animal feed or fertilizer [1,2]. Bioactive peptides from marine resources possess several physiological functions, including antioxidative [3], anticancer [4], antibacterial [5], angiotensin-converting-enzyme (ACE) inhibitory [6], immunomodulatory [7], hepatoprotective [8], and wound healing activities [9]. Extraction of bioactive peptides from marine wastes and by-products might offer new avenues for their utilization, preventing environmental pollution and creating enormous economic benefits [6,10]. MCPs possess several advantages, such as ease of absorption for its lower molecular weight and unique physiological functions (including antioxidation) [3,14], high affinity to calcium [15], antihypertensive [16,17], and wound healing activities [18,19]
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