Abstract
Fish collagen have the potential to be used in biomedical fields due to the biocompatible and biodegradable features. However, how hydrolyzed fish collagen regulate human macrophage has not been clearly unraveled. The aim of the current study is to investigate the effects of hydrolyzed fish collagen on the polarization state of human macrophages. The cell viability of human macrophages treated by hydrolyzed fish collagen were determined using CCK-8 assay. The polarization of human macrophages treated by hydrolyzed fish collagen were detected by quantitative real-time polymerase chain reaction (qRT-PCR). it was found that 0.25,0.5 and 1mg/ml hydrolyzed fish collagen did not alter the cell viability of human macrophages. Further, the data showed that hydrolyzed fish collagen inhibited the expression of M1 macrophage marker IL-ip and TNF-a while enhanced the expression of M2 macrophage marker arginase 1(Arg1) and IL-10. Taken together, the results indicated that treatment with hydrolyzed fish collagen result in a significant shift towards an M2 phenotype for human macrophages.
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