Marine algae Sargassum horneri bioactive factor suppresses proliferation and stimulates apoptotic cell death in human breast cancer MDA-MB-231 cells in vitro

Masayoshi Yamaguchi ,Toru Matsumoto

doi:10.15761/imm.1000127

Abstract

Marine algae Sargassum horneri (S. horneri) bioactive factor has been shown to possess anabolic bone e! ects due to stimulating osteblastic bone formation and suppressing osteoclastogenes. However, the e! ects of S. horneri on cancer cell bone metastasis have not been investigated. is study was undertaken to determine the e! ects of S. horneri bioactive component on human breast cancer MDA-MB-231 bone metastatic cells in vitro. Proliferation of MDA-MB-231 cells was suppressed by culture with S. horneri active component (less than 3000 molecular weight; 10-200 µg/ml) for 5 and 10 days. S. horneri active component was suggested to inhibit G1 and G2/M phase cell cycle arrest in MDA-MB-231 cells using inhibitors of cell-cycle arrest. Moreover, S. horneri active component stimulated apoptotic cell death. is e! ect was prevented in the presence of caspase-3-inhibitor. us, S. horneri active component was found to suppress cell proliferation and stimulate apoptotic cell death in human breast cancer MDA-MB-231 bone metastatic cells in vitro, demonstrating an anticancer e! ect.

Highlights

Breast cancer is by far the most common cancer in women worldwide, and it is associated with a variety of lifestyle choice, such as obesity, later onset of first childbirth, and the use of hormone replacement therapy [1]
We found that S. horneri active component suppresses proliferation and stimulates apoptotic cell death of human breast cancer MDA-MB-231 bone metastatic cells
Number of human breast cancer MDA-MB-231 bone metastatic cells was increased with periods of culture in the presence of Fetal bovine serum (FBS)

Summary

Introduction

Breast cancer is by far the most common cancer in women worldwide, and it is associated with a variety of lifestyle choice, such as obesity, later onset of first childbirth, and the use of hormone replacement therapy [1]. Intake of S. horneri extract was found to prevent bone loss in osteoporosis animal models and in healthy human [8,9,10]. Functional food factor S. horneri extract may be usefulness as an osteogenic factor in preventing osteoporosis in human subjects. S. horneri active component may possess a suppressive effect on bone loss induced by breast cancer cell bone metastasis that is occurred extremely high in breast cancer patients [11,12,13,14,15].

Methods

Results

Conclusion