Abstract
In a continuing effort to understand how cell-generated traction forces are utilised for locomotion, we have applied our modified silicone rubber traction force assay to rapidly locomoting fish epidermal keratocytes executing turns and shape changes, and negotiating obstacles. The resulting maps show that these cells can redistribute tractions from the “steady-state” pattern (previously observed during unobstructed, gliding locomotion), into a variety of transient patterns, with lifetimes of less than 1 minute (Figs. 1-4). The map for a “steady state” locomoting keratocyte shows a maximum traction force density of ~5x10-5 dynes/μm (data not shown). This value was derived from cell-free experiments in which elastic films were manipulated with a pair of micronneedles. Such experiments, in which all forces were known, showed that both the magnitude and direction of traction forces applied to the film could be closely predicted, and that the Young’s modulus of elasticity for the silicone substratum could be calculated. The consequences for understanding the underlying molecular basis for shape change and cell motility from this type of analysis will be discussed.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Proceedings, annual meeting, Electron Microscopy Society of America
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
