Abstract
Three-dimensional (3D) temperature mapping method with high spatial resolution and acquisition rate is of vital importance in evaluating thermal processes in micro-environment. We have synthesized a new temperature-sensitive functional material (Rhodamine B functionalized Polydimethylsiloxane). By performing optical sectioning of this material, we established an advanced method for visualizing the micro-scale 3D thermal distribution inside microfluidic chip with down to 10 ms temporal resolution and 2 ~ 6°C temperature resolution depending the capture parameters. This method is successfully applied to monitor the local temperature variation throughout micro-droplet heat transfer process and further reveal exothermic nanoliter droplet reactions to be unique and milder than bench-top experiment.
Highlights
Three-dimensional (3D) temperature mapping method with high spatial resolution and acquisition rate is of vital importance in evaluating thermal processes in micro-environment
Rhodamine B (RhB) do not have any functional group that can react with PDMS, allyl glycidyl ether (AGE) was chosen as a molecular connector between them (Figure 1a)
The aforementioned temporally-and-spatially temperature mapping results confirmed that our RAP functional material, combining with the confocal microscope, is suitable for mapping 3D thermal propagation in microfluidics
Summary
Three-dimensional (3D) temperature mapping method with high spatial resolution and acquisition rate is of vital importance in evaluating thermal processes in micro-environment. By performing optical sectioning of this material, we established an advanced method for visualizing the micro-scale 3D thermal distribution inside microfluidic chip with down to 10 ms temporal resolution and 2 , 66C temperature resolution depending the capture parameters This method is successfully applied to monitor the local temperature variation throughout micro-droplet heat transfer process and further reveal exothermic nanoliter droplet reactions to be unique and milder than bench-top experiment. Outstanding solvent, thermal stability and photo stability of RAP permits accurate and rapid temperature measurement of local temperature surrounding a nanoliter (nL) droplet trapped inside a RAP chamber This new thermometric methodology is sufficient to characterize vigorous reaction in nL droplet which is much safer and later on recognized that behave distinctively from bench-top size experiments in mL volume
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