Abstract
In living cells, biomolecules are integrated via chemical reactions and transport processes into self-regulated dynamical networks. Through these networks of interactions life-sustaining biological functions, such as gene transcription and signal transduction, emerge at the higher level of organization and at longer spatiotemporal scales. To quantitatively characterize in live cells biomolecules concentration, mobility and properties of their immediate surroundings, we have developed functional Fluorescence Microscopy Imaging (fFMI), a scanning-free time-resolved quantitative confocal fluorescence microscopy imaging technique that integrates massively parallel Fluorescence Correlation Spectroscopy with fluorescence lifetime imaging (Krmpot AJ.
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