Abstract
BackgroundTo this day, the molecular mechanism of endotoxin-induced multi-organ failure has not been completely clarified. This study aimed to construct an miRNA-mRNA regulatory network and identify main pathways and key genes in multi-organ of LPS-mediated endotoxemic mice.MethodsPublic datasets from six mRNA and three miRNA microarray datasets were downloaded from the GEO website to screen final differentially expressed genes (FDEGs) and hub genes in the heart, lung, liver, and kidney of LPS-mediated endotoxemic mice. Functional and pathway enrichment analysis of FDEGs was used to identify the main pathways in multi-organ damage of LPS-treated mice. Finally, hub genes of each organ were intersected to obtain the key genes of multi-organ.ResultsFirstly, 158, 358, 299, and 91 FDEGs were identified in the heart, lung, liver, and kidney, respectively. The pathway enrichment analysis of the FDEGs then showed that the TNF signaling pathway, Toll-like receptor signaling pathway, and some viral-infection-related pathways (influenza A, measles, and herpes simplex) were the main pathways in multi-organ damage of LPS-mediated endotoxemic mice. Moreover, miRNA-mRNA or PPI regulatory networks were constructed based on FDEGs. According to these networks, 31, 34, 34, and 31 hub genes were identified in the heart, lung, liver, and kidney, respectively. Among them, nine key genes (Cd274, Cxcl1, Cxcl9, Icam1, Ifit2, Isg15, Stat1, Tlr2, and Usp18) were enriched in Toll-like receptor signaling pathway and chemokine signaling pathway. Finally, seven potential drugs were predicted based on these key genes.ConclusionThe shared underlying molecular pathways in endotoxin-induced multi-organ damage that have been identified include Toll-like receptor signaling pathway and TNF signaling pathway. Besides, nine key genes (Cd274, Cxcl1, Cxcl9, Icam1, Ifit2, Isg15, Stat1, Tlr2, and Usp18) and seven potential drugs were identified. Our data provide a new sight and potential target for future therapy in endotoxemia-induced multi-organ failure.
Highlights
MATERIALS AND METHODSEndotoxemia is caused by the release of endotoxin from bacteria in the blood and the translocation of endotoxin from intestinal bacteria, which can further cause a systemic inflammatory response and lead to multiple organ failure (Wu et al, 1996; Goligorsky and Sun, 2020)
The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway functional enrichment analysis was showed that these final DEGs (FDEGs) mainly enriched in TNF signaling pathway, NF-kappa B signaling pathway, and Toll-like receptor signaling pathway (Figure 2E)
The KEGG pathway functional enrichment analysis was showed that these FDEGs mainly enriched in TNF signaling pathway, NF-kappa B signaling pathway, Toll-like receptor signaling pathway, cytosolic DNAsensing pathway and FoxO signaling pathway (Figure 5E)
Summary
MATERIALS AND METHODSEndotoxemia is caused by the release of endotoxin from bacteria in the blood and the translocation of endotoxin from intestinal bacteria, which can further cause a systemic inflammatory response and lead to multiple organ failure (Wu et al, 1996; Goligorsky and Sun, 2020). There is no specific drug treatment for endotoxin-mediated multi-organ injury in the clinic, which may lead to high mortality. To this date, the molecular mechanism of endotoxin-induced multi-organ failure has not been completely clarified. It is urgent to study the gene or microRNA (miRNA) regulatory network and common mechanisms of multiple organs in LPS-induced multi-organ damage (LIMOD). To this day, the molecular mechanism of endotoxin-induced multi-organ failure has not been completely clarified. This study aimed to construct an miRNA-mRNA regulatory network and identify main pathways and key genes in multi-organ of LPSmediated endotoxemic mice
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