Mapping the Glyco-Gold Nanoparticles of Different Shapes Toxicity, Biodistribution and Sequestration in Adult Zebrafish

Sivakoti Sangabathuni,Raghavendra Vasudeva Murthy,Preeti Madhukar Chaudhary,Balamurugan Subramani,Suraj Toraskar,Raghavendra Kikkeri

doi:10.1038/s41598-017-03350-3

Sivakoti Sangabathuni, Raghavendra Vasudeva Murthy + Show 4 more

Open Access

https://doi.org/10.1038/s41598-017-03350-3

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Abstract

Glyconanotechnology offers a broad range of applications across basic and translation research. Despite the tremendous progress in glyco-nanomaterials, there is still a huge gap between the basic research and therapeutic applications of these molecules. It has been reported that complexity and the synthetic challenges in glycans synthesis, the cost of the high order in vivo models and large amount of sample consumptions limited the effort to translate the glyco-nanomaterials into clinical applications. In this regards, several promising simple animal models for preliminary, quick analysis of the nanomaterials activities has been proposed. Herein, we have studied a systematic evaluation of the toxicity, biodistribution of fluorescently tagged PEG and mannose-capped gold nanoparticles (AuNPs) of three different shapes (sphere, rod, and star) in the adult zebrafish model, which could accelerate and provide preliminary results for further experiments in the higher order animal system. ICP-MS analysis and confocal images of various zebrafish organs revealed that rod-AuNPs exhibited the fast uptake. While, star-AuNPs displayed prolong sequestration, demonstrating its potential therapeutic efficacy in drug delivery.

Highlights

IntroductionKovriznych et al have shown that zebrafish model can be used for optimize the toxicity of the different types of nanoparticles[24,25,26,27]
To assess the interplay between the shape and carbohydrate-mediated interactions in the zebrafish model, AuNPs were synthesized by using PEG or mannose and fluorescein linker
The required mannose-linker was synthesized as described in the literature[28]

Summary

Introduction

Kovriznych et al have shown that zebrafish model can be used for optimize the toxicity of the different types of nanoparticles[24,25,26,27]. The shape dependent gold nanoparticles toxicity, biodistribution and sequestration has not studied well so far in zebrafish model. We report how different shapes of PEG and mannose capped goldnanoparticles (AuNPs) influence the toxicity, uptake and clearance in the zebrafish model. It has been shown that shape of the glyco-gold nanoparticles significantly influences carbohydrate-mediated bacterial adhesion and endocytosis in mammalian cells[28]. Deciphering the role of different shapes of gold-nanoparticles in in vivo system undoubtedly results in designing better glycoprobes to target or inhibit the carbohydrate-protein interactions (CPI)

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