Abstract
BackgroundAnthocyanins are flavonoid pigments that are responsible for purple coloration in the stems and leaves of a variety of plant species. Anthocyaninless (anl) mutants of Brassica rapa fail to produce anthocyanin pigments. In rapid-cycling Brassica rapa, also known as Wisconsin Fast Plants, the anthocyaninless trait, also called non-purple stem, is widely used as a model recessive trait for teaching genetics. Although anthocyanin genes have been mapped in other plants such as Arabidopsis thaliana, the anl locus has not been mapped in any Brassica species.ResultsWe tested primer pairs known to amplify microsatellites in Brassicas and identified 37 that amplified a product in rapid-cycling Brassica rapa. We then developed three-generation pedigrees to assess linkage between the microsatellite markers and anl. 22 of the markers that we tested were polymorphic in our crosses. Based on 177 F2 offspring, we identified three markers linked to anl with LOD scores ≥ 5.0, forming a linkage group spanning 46.9 cM. Because one of these markers has been assigned to a known B. rapa linkage group, we can now assign the anl locus to B. rapa linkage group R9.ConclusionThis study is the first to identify the chromosomal location of an anthocyanin pigment gene among the Brassicas. It also connects a classical mutant frequently used in genetics education with molecular markers and a known chromosomal location.
Highlights
Anthocyanins are flavonoid pigments that are responsible for purple coloration in the stems and leaves of a variety of plant species
Microsatellite markers We tested a total of 138 microsatellite markers for amplification and polymorphism in the rapid-cycling Brassica rapa (RBr) test population
Most (122 out of 138) were from B. rapa, but a few from other Brassica species were tested since the sequence homology between Brassicas in the U Triangle allowed for analysis of microsatellites first identified in B. napus, B. oleracea and B. nigra [16,17,18]
Summary
Anthocyanins are flavonoid pigments that are responsible for purple coloration in the stems and leaves of a variety of plant species. Anthocyaninless (anl) mutants of Brassica rapa fail to produce anthocyanin pigments. Anthocyanin genes have been mapped in other plants such as Arabidopsis thaliana, the anl locus has not been mapped in any Brassica species. They have been cited as contributing to protection from photoinhibition [1], protection from UVB light [2] and modification of captured light quality and quantity [3]. In the model organism Arabidopsis thaliana, study of anthocyaninless mutants led to the discovery of (page number not for citation purposes)
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