Mapping quantitative trait loci associated with stripe rust resistance from the Canadian wheat cultivar ‘AAC Innova’

Abstract

Stripe rust, caused by Puccinia striiformis Westend. f.sp. tritici Erikss. (Pst), is one of the most devastating diseases of wheat (Triticum aestivum L.) globally. Exploring and utilizing new sources of resistance is essential for breeding resistant wheat cultivars. Thus, a doubled haploid population (n = 291) derived from the cross ‘AAC Innova’/‘AAC Proclaim’ was evaluated to dissect the genetics of resistance in the cultivar ‘AAC Innova’. This population was evaluated for stripe rust severity in disease nurseries at Creston, British Columbia (in 2016 and 2020), and Lethbridge, Alberta (in 2016, 2017 and 2020), and genotyped using the wheat 90 K SNP assay. A high-density genetic map was constructed which consisted of 7112 SNP markers with an average marker interval of 2.3 cM. Quantitative trait loci (QTL) mapping identified one major (QYr.lrdc-2A) and 10 minor effect (QYr.lrdc-2B.1, QYr.lrdc-2B.2, QYr.lrdc-2B.3, QYr.lrdc-2B.4, QYr.lrdc-2D, QYr.lrdc-3B, QYr.lrdc-5A, QYr.lrdc-5B, QYr.lrdc-5D and QYr.lrdc-7D) loci. The QTL QYr.lrdc-2A was consistently effective against Pst races across all environments and explained up to 33.0% of the phenotypic variation. Other QTLs were either less consistent or environment specific. ‘AAC Innova’ contributed stripe rust resistance alleles for most of the QTLs except for QYr.lrdc-2D, QYr.lrdc-5A and QYr.lrdc-7D. The QTLs identified in this study should be valuable for diversifying the sources of genetic resistance against current and new virulent races of Pst. In particular, the ‘AAC Innova’-derived QTL QYr.lrdc-2A could be a valuable source of resistance to incorporate into commercial cultivars and combine with other genes using the associated SNP markers identified in this study.