Mapping of the Insulin Promoter Factor I Gene ( Ipf1) to Distal Mouse Chromosome 5

Abstract

We have identified sequence variants in the 3′ untranslated region of the insulin promoter factor 1 ( Ipf1) cDNA sequence in mice and used a PCR-based oligonucleotide hybridization assay to map the Ipf1 gene to distal mouse chromosome (Chr) 5. An identical 12-bp insertion in the 3′ untranslated region of the mouse Ipf1 gene sequence is present in Mus spretus and Mus m. musculus Czech II mice, two feral strains used for meiotic mapping by backcross analysis. The human IPF1 gene homolog would be expected to map to either chromosome 13q12-q14 or 7pter-q21 based on homology of synteny of other loci in this region of mouse Chr 5. Given its genetic map position, the Ipf1 gene may be part of a cassette of homeodomain-containing transcription factors involved in the development of the pancreas and other foregut-derived organs.