Abstract
We have isolated several new cDNA clones that are differentially expressed in retina from a human retina cDNA library using a new combined method of subtractive hybridization and differential hybridization. Among these cDNAs, we found that cDNA clone BA7 was identical to the previously reported Pmel 17 cDNA in its nucleotide sequence. Pmel 17 mRNA is preferentially expressed in melanocytes, and the protein expressed in Escherichia coli cross-reacts with anti-tyrosinase antibodies. The mouse Pmel 17 gene was mapped to the pter-q21 region of human chromosome 12. In this paper, we present evidence precisely locating the human Pmel 17 gene to chromosome 12q13-q14. 7 refs., 1 fig.
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