Mapping of spinal cord circuits controlling the bladder and external urethral sphincter functions in the rabbit.

Abstract

A primary purpose of this study was to evaluate the rabbit as a model for studying the spinal circuitry controlling the bladder emptying. We aimed to map the locations of the neuronal circuitry controlling the external urethral sphincter (EUS) and the detrusor by stimulating at different spinal cord locations with a microelectrode, while recording the responses from these muscles. Spinal cord microstimulation was performed in the intermediate zone of the gray matter at the L7-S4 spinal cord levels in eight rabbits with empty and full bladders. Bladder activity was measured as intravesical pressure (IVP) changes and EUS activity was measured via electromyographic (EMG) electrodes positioned within the urethra. Under both bladder conditions, EUS activation was produced from similar locations in the spinal cord comprising a continuous area in the intermediate zone of the S2-S3 spinal cord. This region extended 25 mm in the rostrocaudal dimension, at least 1 mm lateral to the midline, and 0.5-1 mm in the dorsoventral dimension at a depth of 2-3 mm beneath the dorsal surface. No locations in the intermediate zone produced EUS inhibition. The S2-S3 spinal region, stimulation of which produced the strongest EUS activation, also produced modest bladder contractions. Overall, the results indicate that spinal cord networks controlling bladder and EUS activation in the rabbit are overlapping and clustered into columns extending rostrocaudally. The lack of spinal locations producing EUS inhibition and large bladder contractions make the rabbit an unattractive model for studies of neuroprosthetic spinal control of micturition.