Abstract
Methods Naive male mauritian cynomolgus macaques were immunized with auxo-GTU-MultiSIV DNA encoding Gag, Nef, Rev and Tat (n=14) or by a combination of auxo-GTUMultiSIV DNA and auxo-GTU-SIV-vifvprvpx (n=12) by the intradermal route followed by electroporation. MHC class I and class II haplotypes were determined by microsatellite analysis. T-cell epitopes were mapped by IFN-g ELISPOT in PBMC by using matrix of 15-mer (overlapping by 11) SIVmac239 Gag, Nef, Rev, Tat, Vif, Vpx and Vpr peptides. Sequence and length of candidate epitopes were further optimized.
Highlights
Intradermal immunization with electroporation using auxo-GTU-MultiHIV DNA encoding Gag, Nef, Rev and Tat induces strong and sustained T cell responses in cynomolgus macaques
Mapping of SIVmac T-cell epitopes in cynomolgus macaques immunized with auxoGTU-MultiSIV DNA by the intradermal route followed by electroporation
Naïve male mauritian cynomolgus macaques were immunized with auxo-GTU-MultiSIV DNA encoding Gag, Nef, Rev and Tat (n=14) or by a combination of auxo-GTUMultiSIV DNA and auxo-GTU-SIV-vifvprvpx (n=12) by the intradermal route followed by electroporation
Summary
Intradermal immunization with electroporation using auxo-GTU-MultiHIV DNA encoding Gag, Nef, Rev and Tat induces strong and sustained T cell responses in cynomolgus macaques. We used an equivalent vaccine encoding SIVmac239 antigens (Gag, Nef, Rev, Tat, Vif, Vpx and Vpr) and we described the breath of this T cell response
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
