Mapping of main and hidden epistatic QTL effects in spring wheat population using medium parental FHB resistance

Abstract

Fusarium head blight (FHB) is a devastating disease lowering the yield and quality of wheat. Intensive use of limited allelic pools critically affects wheat resistance, while advancing pathogenic alleles. This study presents new alleles raised from moderate rather than elite crosses. ‘Parshall’ has indigenous moderate resistance that was crossed with the moderate susceptible ‘Reeder’ and developed the 110 RIL population (noted as PR) that was tested across three states and years in the USA, under open-field and greenhouse conditions. Heading- and FHB-related traits of incidence, severity, and index were assessed. A genetic map (1417 cM) of SNP/DArT markers was generated. Composite interval mapping produced 45 QTL. Permutated LOD assessment identified two consistent QTL (4AL and 4BL) of type I and II resistance. Significant markers at the 4A QTL showed correlation with previous FHB resistance, while at the 4B QTL with pathogen responsive regions. The 1- and 2-D genome scans identified 16 QTL and six pairs of interacting markers for resistance, with additive effects between 0.17% and 2.19%; respectively. Additionally, additive × environment interaction was between 0.04% and 3.18% confirming the high environmental effect. Three pairs of QTL with additive × additive effects between 0.15% and 0.30% were revealed by FHB epistasis. However, the additive × additive environmental interaction at these three loci varied between 0.03% and 1%, confirming the G × E effect on FHB resistance. Hidden heritable epistasis was discovered in PR-population for FHB resistance. PR-alleles will help breeders develop lines mitigating epidemic environments and changeable climates, to boost yield facing hanger, food scarce, and secure worldwide penurious economies.