Abstract
Here, we show that semiconductor-based sequencing technology can be used to map mammalian replication domains, chromosomal units with similar DNA replication timing. Replicating DNA purified from mammalian cells was successfully sequenced by the Ion Torrent platform. The resultant replication domain map of mouse embryonic stem cells is comparable to those obtained by the conventional microarray-based method.
Highlights
AB Library Builder System (Thermo Fisher Scientific, #4,477,683) and Ion Xpress Barcode Adapters 1–16 Kit (Thermo Fisher Scientific, #4,477,683)
We found that obtained replication domain map of 46C embryonic stem (ES) cells is almost identical to the previous comparative genomic hybridization (CGH) microarray-based replication domain map from the same cell line
A representative ES-cell specific early replicating domain identified in the previous study[4,5] is detected in the Ion Proton-based replication domain map (Figure 1(d))
Summary
AB Library Builder System (Thermo Fisher Scientific, #4,477,683) and Ion Xpress Barcode Adapters 1–16 Kit (Thermo Fisher Scientific, #4,477,683). Sequencing by Ion Proton d 2 46C ESC (Ion Proton) 46C ESC (Ion Proton) 46C ESC (CGH array) Sequencing was performed on the Ion PITM Chip kit v3 chip using Ion Proton semiconductor sequencer (Thermo Fisher Scientific).
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