Mapping Male Fertility Gene with SSR Markers in Parents of Cytoplasmic- Nuclear Male-Sterile Line NJCMS3A in Soybean

Abstract

Utilization of the three lines [cytoplasmic-nuclear male-sterile(CMS) line,maintainer line,and restorer line] in producing hybrids with heterosis has been very successful in a number of crops including soybean.The soybean CMS line NJCMS3A was developed through six consecutive backcross generations with N21566 as cytoplasm source and N21249 as nuclear donor.The objective of our study was to reveal the genetic mechanism and mapping the male fertility gene in the two parents of the CMS line NJCMS3A,i.e.N21566 and N21249.The male fertility of(N21566×N21249) F_2 and BC_1F_1 showed that the segregation ratio of fertile to sterile plants fitted the expected ratios of 3:1 and 1:1,respectively,indicating one pair of gene conferring male fertility in the parents of NJCMS3A with dominance allele in N21249 being male fertile.This gene might be one of the restorer genes for the male sterility of NJCMS3A.The mapping results on BC_1F_1 and F_2 showed that out of the 793 randomly selected SSR markers,Satt331,CSSR133 and Satt477 on linkage group O were linked to the male fertility gene Rf with their genetic distances of 8.1～10.4,11.4～16.4,and 13.3～19.2 cM,respectively,in a same order and its location between Satt331 and Satt477.