Mapping Genes of Complex Disease in Genetic Isolates of Daghestan

Abstract

Original results of the analysis of genetic linkage between some genomic markers and two complex clinical phenotypes, schizophrenia and mental retardation, in pedigrees from Daghestan genetic isolates are described. Interpopulation differences in the epidemiology of the complex phenotypes were studied and in their genetic linkage was demonstrated. These differences are evidently related to the genetic structure of the isolates determined by their demographic history. The epidemiological index MR characterizing the lifetime morbid risk of schizophrenia varies in the Daghestan isolates studied from 0 to 4.95%, which is almost five times higher than the average worldwide population rate, 1%. Comparative genetic mapping in different isolates permitted determination of the most probable genetic linkages and associations of loci in chromosomal regions 17p11.1–12, 3q13.3, and a locus from 22q with schizophrenia and locus 12q23 with mental retardation. There is evidence that this approach is effective for detailed study of the relationship between the genetic (allele and locus) and clinical heterogeneity of complex diseases, which favors successful identification of the genes determining them. The study of linkage disequilibrium (LD) in genetic isolates of Daghestan ethnic populations (which have a common genetic background) may be an effective methodological approach for revealing the numerous contradictory results of mapping of genes of the same complex disease performed by different researchers in different regions of the world.