Abstract
Rice black-streaked dwarf virus (RBSDV) disease is one of the most destructive viral diseases that threatens rice production in China. Breeding of resistant cultivars through multi-gene pyramiding is considered to be an effective way to control the disease, but few resistance genes have been characterized to date. In the present study, we identified T1012, a BC2F6 line from a cross of the japonica variety ‘Wuyujing3’ (recipient) and the indica variety ‘Dular’ (donor), that had improved resistance to RBSDV disease in a field test, and 140 backcross inbred lines (BILs) derived from a cross between T1012 and ‘Wuyujing3’ were developed using marker-assisted selection. Genetic analysis showed that the resistance of T1012 to RBSDV disease was controlled by quantitative trait loci (QTLs). Two QTLs for RBSDV disease resistance located on chromosomes 1 and 4, qRBSDV-1 and qRBSDV-4, were identified, and qRBSDV-4 was repeatedly detected in two environments. Compared to ‘Wuyujing3’, the line containing only the substitution segment covering qRBSDV-4 exhibited significantly decreased disease incidence, indicating that qRBSDV-4 is a reliable resistance QTL with a high breeding value. Furthermore, two linked QTLs, qRBSDV-4-1 and qRBSDV-4-2, were identified within the interval containing qRBSDV-4. The QTLs identified here will provide a useful resource for breeding RBSDV-resistant rice cultivars through marker-assisted selection and establish a foundation for the cloning of RBSDV disease resistance genes.
Highlights
Rice black-streaked dwarf virus (RBSDV) disease is one of the most destructive viral diseases that threatens rice production in China
The QTLs identified here will provide a useful resource for breeding RBSDV-resistant rice cultivars through marker-assisted selection and establish a foundation for the cloning of RBSDV disease resistance genes
Rice black-streaked dwarf virus disease is a viral disease caused by the rice black-streaked dwarf virus (RBSDV: Fijivirus; Rheoviridae), which is spread by the small brown planthopper (Laodelphax striatellus Fallén; SBPH)
Summary
Rice black-streaked dwarf virus (RBSDV) disease is one of the most destructive viral diseases that threatens rice production in China. RBSDV disease was first reported in China in the 1960s, and the recent reemergence of RBSDV has caused severe yield losses in China and other East Asian countries (Sun et al 2013, Ministry of Agriculture of PR China 2010). In rice production, this virus is controlled by spraying with pesticides to control the SBPH insect vector (Sun et al 2017). Identification of resistant rice germplasm and mapping RBSDV resistance genes are of great significance for controlling RBSDV disease
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
