Abstract

Vesicular stomatitis virus synthesizes a leader RNA in addition to the five monocistronic mRNA species in vitro. The leader RNA molecule appears to be an initiated RNA molecule since it is polyphosphorylated at its 5′-terminus. In addition, uv inactivation studies on purified virions suggest that leader RNA is synthesized prior to mRNA synthesis. The leader RNA is coded for at the 3′-end of genome RNA since purified leader RNA will protect the 3′-terminal region of the genome RNA from RNase digestion. Kinetic studies show that leader RNA and mRNA coding for the N protein anneal to 42 S genome RNA at the same rate, indicating that the leader RNA is coded for only once on the 42 S genome RNA. In the absence of UTP, an oligonucleotide is synthesized in vitro which contains the same 5′-terminal sequence as the leader RNA. These results indicate that the synthesis of the leader RNA is the first event during in vitro RNA transcription by vesicular stomatitis virus.

Full Text
Published version (Free)

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call