Abstract
Anthracnose is a destructive disease of the common bean (Phaseolus vulgaris L.). The Andean cultivar Hongyundou has been demonstrated to possess strong resistance to anthracnose race 81. To study the genetics of this resistance, the Hongyundou cultivar was crossed with a susceptible genotype Jingdou. Segregation of resistance for race 81 was assessed in the F2 population and F2:3 lines under controlled conditions. Results indicate that Hongyundou carries a single dominant gene for anthracnose resistance. An allele test by crossing Hongyundou with another resistant cultivar revealed that the resistance gene is in the Co-1 locus (therefore named Co-1HY). The physical distance between this locus and the two flanking markers was 46 kb, and this region included four candidate genes, namely, Phvul.001G243500, Phvul.001G243600, Phvul.001G243700 and Phvul.001G243800. These candidate genes encoded serine/threonine-protein kinases. Expression analysis of the four candidate genes in the resistant and susceptible cultivars under control condition and inoculated treatment revealed that all the four candidate genes are expressed at significantly higher levels in the resistant genotype than in susceptible genotype. Phvul.001G243600 and Phvul.001G243700 are expressed nearly 15-fold and 90-fold higher in the resistant genotype than in the susceptible parent before inoculation, respectively. Four candidate genes will provide useful information for further research into the resistance mechanism of anthracnose in common bean. The closely linked flanking markers identified here may be useful for transferring the resistance allele Co-1HY from Hongyundou to elite anthracnose susceptible common bean lines.
Highlights
Common bean (Phaseolus vulgaris L.) is one of the most important and widely cultivated grain legumes, in China
Co-1, Co-x and Co-w belong to the Andean gene pool, and the others belong to the Mesoamerican gene pool
Sporulation never occurred from the inoculated leaves of the resistant plants, whereas abundant spores were collected from the inoculated leaves of the susceptible plants (Fig 1E and 1F)
Summary
Common bean (Phaseolus vulgaris L.) is one of the most important and widely cultivated grain legumes, in China. Magn) Scrib has become a major cause of reduced dry bean production in China. Twenty-three related anthracnose resistance genes have been identified by the ‘Co’ symbol, including Co-1 to Co-7, Co-8, Co-9 to Co-15, Co-17, Co-u to Co-z, and CoPv09 [3,4,5]. Among these genes, Co-1, Co-x and Co-w belong to the Andean gene pool, and the others belong to the Mesoamerican gene pool. Most of the anthracnose resistance genes have been located on common bean linkage maps, and flanking markers were determined. Campa et al identified a new gene CoPv09 on Pv09 [3]
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