Abstract
Previously, the abundance of p42/44 and p38 MAPK proteins had been shown to be higher in arteries of 1- to 2-week-old compared to 2- to 3-month-old rats. However, the role of MAPKs in vascular tone regulation in early ontogenesis remains largely unexplored. We tested the hypothesis that the contribution of p42/44 and p38 MAPKs to the contraction of peripheral arteries is higher in the early postnatal period compared to adulthood. Saphenous arteries of 1- to 2-week-old and 2- to 3-month-old rats were studied using wire myography and western blotting. The α1-adrenoceptor agonist methoxamine did not increase the phosphorylation level of p38 MAPK in either 1- to 2-week-old or 2- to 3-month-old rats. Accordingly, inhibition of p38 MAPK did not affect arterial contraction to methoxamine in either age group. Methoxamine increased the phosphorylation level of p42/44 MAPKs in arteries of 2- to 3-month-old and of p44 MAPK in 1- to 2-week-old rats. Inhibition of p42/44 MAPKs reduced methoxamine-induced contractions in arteries of 2- to 3-month-old, but not 1- to 2-week-old rats. Thus, despite a high abundance in arterial tissue, p38 and p42/44 MAPKs do not regulate contraction of the saphenous artery in the early postnatal period. However, p42/44 MAPK activity contributes to arterial contractions in adult rats.
Highlights
IntroductionPostnatal maturation is associated with growth and development of different organs and systems, including the circulatory system
As before [3], the content of p38 and p42/44 MAPKs was considerably higher in arteries of 1- to 2-week-old rats in comparison p42/44 MAPKs was considerably higher in arteries of 1- to 2-week-old rats in comparison to 2- to 3-month-old rats (Figure 1a–c)
MAPKs in peripheral muscular type arteries of rats in early postnatal ontogenesis, they do not participate in the regulation of their contractile responses to an agonist of α1 adrenoceptors
Summary
Postnatal maturation is associated with growth and development of different organs and systems, including the circulatory system. The development of the vascular system is a complex process and occurs at different structural and functional levels, from the systemic (maturation is accompanied by an increase in blood pressure [1,2]). To the molecular (maturational changes in protein expression and of the mechanisms of vascular smooth muscle contractility control [3,4,5,6]). Functional remodeling of vascular smooth muscle during postnatal development includes (but is not limited to) an increase in the Ca2+ -dependent control of contractility and a decrease in the Ca2+ -sensitivity of the contractile apparatus [3].
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