Abstract
BackgroundMitogen Activated Protein Kinase (MAPK) cascade is a fundamental pathway in organisms for signal transduction. Though it is well characterized in various plants, there is no systematic study of this cascade in tea.ResultIn this study, 5 genes of Mitogen Activated Protein Kinase Kinase (MKK) and 16 genes of Mitogen Activated Protein Kinase (MPK) in Camellia sinensis were found through a genome-wide search taking Arabidopsis thaliana as the reference genome. Also, phylogenetic relationships along with structural analysis which includes gene structure, location as well as protein conserved motifs and domains, were systematically examined and further, predictions were validated by the results. The plant species taken for comparative study clearly displayed segmental duplication, which was a significant candidate for MAPK cascade expansion. Also, functional interaction was carried out in C. sinensis based on the orthologous genes in Arabidopsis. The expression profiles linked to various stress treatments revealed wide involvement of MAPK and MAPKK genes from Tea in response to various abiotic factors. In addition, the expression of these genes was analysed in various tissues.ConclusionThis study provides the targets for further comprehensive identification, functional study, and also contributed for a better understanding of the MAPK cascade regulatory network in C. sinensis.
Highlights
Mitogen Activated Protein Kinase (MAPK) cascade is a fundamental pathway in organisms for signal transduction
This study provides the targets for further comprehensive identification, functional study, and contributed for a better understanding of the MAPK cascade regulatory network in C. sinensis
Identification of MAPK genes in Camellia sinensis In order to search for kinase domain in Tea Plant Information Archive (TPIA), the Hidden Markov Model (HMM) of the kinase domains (PF00069) was utilized
Summary
Mitogen Activated Protein Kinase (MAPK) cascade is a fundamental pathway in organisms for signal transduction. Though it is well characterized in various plants, there is no systematic study of this cascade in tea. Apart from the biotic and abiotic stresses, modulation of the MAPK-pathway trigger mechanism can depend on the ROS signalling [9, 10]. These reactive oxygen species are formed when oxygen reduces partially and they can be produced intracellularly as well as apoplastically [10]. Though the mechanism of activation and the downstream targets of the MAPK pathways are not recognized, ROS-induced activation of MAPKs seems to play a central role for mediating cellular responses to various stresses [11]
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