Abstract
Rice (Oryza sativa L.) production, essential for global food security, is threatened by the brown planthopper (BPH). The breeding of host-resistant crops is an economical and environmentally friendly strategy for pest control, but few resistance gene resources have thus far been cloned. An indica rice introgression line RBPH54, derived from wild rice Oryza rufipogon, has been identified with sustainable resistance to BPH, which is governed by recessive alleles at two loci. In this study, a map-based cloning approach was used to fine-map one resistance gene locus to a 24kb region on the short arm of chromosome 6. Through genetic analysis and transgenic experiments, BPH29, a resistance gene containing a B3 DNA-binding domain, was cloned. The tissue specificity of BPH29 is restricted to vascular tissue, the location of BPH attack. In response to BPH infestation, RBPH54 activates the salicylic acid signalling pathway and suppresses the jasmonic acid/ethylene-dependent pathway, similar to plant defence responses to biotrophic pathogens. The cloning and characterization of BPH29 provides insights into molecular mechanisms of plant-insect interactions and should facilitate the breeding of rice host-resistant varieties.
Highlights
Rice (Oryza sativa L.), an important staple food for >3 billion people worldwide (Khush, 1997), is currently beset by multiple threats
The BPH29 locus region was narrowed down to 24 kb on the short arm of chromosome 6, and the location of BPH29 was confirmed by genetic analysis in this target region (Figs 1, 2; Supplementary Fig. S2 at JXB online)
brown planthopper (BPH) resistance is known to be monogenically controlled in most resistant sources, and little progress has been made on recessive resistance genes compared with dominant ones (Huang et al, 2013)
Summary
Rice (Oryza sativa L.), an important staple food for >3 billion people worldwide (Khush, 1997), is currently beset by multiple threats. The brown planthopper (BPH), Nilaparvata lugens Stål (Homoptera: Delphacidae), is a monophagous herbivore of rice and causes heavy economic losses throughout Asia. These insects feed mainly on stems, and account for 28% of total plant dry matter reduction (Sōgawa, 1994). Feeding by lowdensity BPH populations can reduce rice yields Development of insect-resistant crop varieties is of interest as an economical, environmentally friendly alternative strategy (Khush, 2001)
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