Abstract
BackgroundMicrotubule-associated proteins 7(MAP7) was reported to be engaged into the function of neuronal function. The function of MAP7 in human cervical cancer (CC) was unknown. We aimed to uncover the function and mechanism of MAP7 on CC.MethodsWe applied qRT-PCR, western blot and immunochemistry to detect the expression difference between normal tissue and CC. In vitro, we establish MAP7 stable knocking down and overexpression cell lines and investigated the function and underlying mechanism of MAP7 in CC.ResultsBoth mRNA and protein of MAP7 were upregulated in CC compared with the normal tissue. MAP7 was correlated with the clinical stage and tumor size and lymph node metastasis. MAP7 promotes the invasion and migration of CC cell lines. We next detected EMT pathway and autophagy associated pathway. MAP7 promotes the EMT through modulating the autophagy.ConclusionTaken above, our results showed that MAP7 promotes the migration and invasion and EMT through modulating the autophagy.
Highlights
Microtubule-associated proteins 7(MAP7) was reported to be engaged into the function of neuronal function
Microtubule associated protein 7 (MAP7) was upregulated in cervical cancer We scanned the most authoritative database in cancerTCGA database and identified MAP7 was upregulated in 306 Cervical cancer compared with normal tissue (Fig. 1a)
The number of normal tissues in TCGA database was small so we detected the mRNA level of MAP7
Summary
Microtubule-associated proteins 7(MAP7) was reported to be engaged into the function of neuronal function. The function of MAP7 in human cervical cancer (CC) was unknown. We aimed to uncover the function and mechanism of MAP7 on CC. Cervical cancer (CC) is the second most common gynecological carcinoma worldwide [1]. Microtubule was responsible for vital cellular processes such as cell division and differentiation [4, 5]. Microtubule associated protein 7 (MAP7) was crucial in the regulating of microtubule bundling and dynamics. MAP7 was reported to be engaged into the function of neuron cell [6]. MAP7 was reported to be upregulated and correlated with the prognosis of cancers [7, 8]. The function and mechanism of MAP7 in CC was still unknow
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