MAP65-1 is required for the depolymerization and reorganization of cortical microtubules in the response to salt stress in Arabidopsis

Abstract

Microtubules (MTs) are highly dynamical structures that play crucial roles in plant development and in response to environmental signals and stress conditions. MT-associated proteins (MAPs) play important roles in regulating the organization of MT arrays. MAP65 is a family of plant MT-bundling proteins. Here, we determined the role of MAP65-1 in the response to salt stress. MAP65-1 is involved not only in regulating the depolymerization, but also in the following reorganization of cortical MTs in salt stress responses. In addition, the depolymerization of the cortical MTs affected the survival of seedlings during salt stress, and map65-1 mutants had enhanced salt hypersensitivity levels. MAP65-1 interacted with mitogen-activated protein kinase (MPK) 3 and 6; however, only the mpk6 mutant exhibited hypersensitivity to salt stress, and MPK6 was involved in regulating the salt stress-induced depolymerization of cortical MTs. Thus, MAP65-1 plays a critical role in the response to salt stress and is required for regulating the rapid depolymerization and reorganization of cortical MTs. MAP65-1 interacts with MPK6, not MPK3, affecting the MT’s dynamic instability which is critical for plant salt-stress tolerance.