Abstract
Plants use surface receptors to perceive information about many aspects of their local environment. These receptors physically interact to form both steady state and signalling competent complexes. The signalling events downstream of receptor activation impact both plant developmental and immune responses. Here, we present a comprehensive study of the physical interactions between the extracellular domains of leucine-rich repeat receptor kinases (LRR-RKs) in Arabidopsis. Using a sensitized assay, we tested reciprocal interactions among 200 of the 225 Arabidopsis LRR-RKs for a total search space of 40,000 interactions. Applying a stringent statistical cut-off and requiring that interactions performed well in both bait-prey and prey-bait orientations resulted in a high-confidence set of 567 bidirectional interactions. Additionally, we identified a total of 2,586 unidirectional interactions, which passed our stringent statistical cut-off in only one orientation. These datasets will guide further investigation into the regulatory roles of LRR-RKs in plant developmental and immune signalling decisions.
Highlights
Background & SummaryDue to their sessile nature, plants must be able to accurately sense ever-changing signals from their local growth environment
The best-studied examples of leucine-rich repeat receptor kinases (LRR-RKs) activation initially involve a ligand binding to the extracellular domain (ECD) of a cognate receptor
This induces the physical interaction between the ECDs of the receptor LRR-RK and a co-receptor LRR-RK to form a signalling competent complex[4]
Summary
Due to their sessile nature, plants must be able to accurately sense ever-changing signals from their local growth environment. We present the data from that study in its most expanded form, including the data used for the published analyses, an additional analysis that identifies a set of interactions that are found in only one orientation, and the raw data needed for the implementation of other normalization and hit-calling protocols These data provide unique opportunities to formulate experimentally testable hypotheses aimed at understanding further how physical interactions in LRR-RK complexes control plant developmental and immune responses. We chose an extremely stringent cut-off to build a highconfidence interaction network including the most reliable bidirectional interactions We used these data to: i- assign biological function to previously uncharacterized receptors, and ii- demonstrate that the interconnectivity of physical interactions between LRR-RKs is a requisite to appropriately transduce a complex range of environmental signals to the plant[9]. Interactions occurring only in one of the bait-prey or prey-bait orientations (unidirectional) have the potential to yield further biological insights
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