Abstract
The great properties of human mesenchymal stromal cells (hMSCs) make these cells an important tool in regenerative medicine. Because of the limitations of hMSCs derived from the bone marrow during isolation and expansion, hMSCs derived from the umbilical cord stroma are a great alternative to overcome these issues. For a large expansion of these cells, we performed a process transfer from static culture to a dynamic system. For this reason, a microcarrier selection out of five microcarrier types was made to achieve a suitable growth surface for the cells. The growth characteristics and metabolite consumption and production were used to compare the cells growth in 12-well plate and spinner flask. The goal to determine relevant process parameters to transfer the expansion process into a stirred tank bioreactor was achieved.
Highlights
Mesenchymal stromal cells (MSCs) play an important role in regenerative medicine, for cell therapy or tissue engineering [1,2,3,4]
MSCs are found in other tissues beside the marrow cavity; for example, they can be found in blood or adipose tissue [8], dermis, muscle, dental pulp, umbilical cord blood, placenta, perivascular areas, amniotic fluid, and tissues surrounding the umbilical cord vessels, called Wharton’s jelly [8, 9, 16]
The hUC MSCs were sent for karyotype analysis at passage 5, and the karyotype was normal. These results indicate that dynamically produced hUC MSCs meet International Society for Cellular Therapy (ISCT) MSC minimal definition
Summary
Mesenchymal stromal cells (MSCs) play an important role in regenerative medicine, for cell therapy or tissue engineering [1,2,3,4]. This importance is based on properties of these cells. MSCs have the capacity to differentiate to osteoblasts, adipocytes, and chondrocytes, which classifies MSCs as multipotent stromal cells [5,6,7,8]. The immune properties of MSCs give these cells an important role to treat immunological disorders, such as graft-versus-host disease [9]. The advantage of isolation of MSCs from the umbilical cord is that collection is safe and painless to mother and child, in contrast to the invasive and painful extraction of MSCs from the bone marrow
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